Always run a gradient PCR on your first two "A grade" candidates from PP6—even the best software cannot account for weird GC clamps in your specific DNA prep. Need help troubleshooting a primer-dimer issue? Run your sequence through PP6’s "Cross-Dimer" analysis and look for a ΔG below -6.0 kcal/mol.
Always run a gradient PCR on your first two "A grade" candidates from PP6—even the best software cannot account for weird GC clamps in your specific DNA prep. Need help troubleshooting a primer-dimer issue? Run your sequence through PP6’s "Cross-Dimer" analysis and look for a ΔG below -6.0 kcal/mol.
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